ferulic acid protects pc12 neurons against hypoxia by inhibiting the p-mapks and cox-2 pathways
نویسندگان
چکیده
objective(s):hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. here, the protective effects of ferulic acid (fa) on hypoxia-induced neurotoxicity in pc12 cells were evaluated. materials and methods:we investigated the effect of fa on pc12 cells subjected to hypoxia stress, in vitro. results:fa increased cell viability, prevented membrane damage (ldh release), scavenged free radicals, increased superoxide dismutase (sod) activity, and attenuated the elevation of intracellular free ca2+, lipid peroxidation, apoptosis (evaluated by tunel staining) and pge2 production in hypoxia-stressed pc12 cells. mapks were activated during hypoxia. fa reduced p-p38 mapk, caspase-3, and cox-2 activation which correlated well with diminished ldh release in pc12 cells under hypoxia. furthermore, fa reduced lipid peroxidation in pc12 cells subjected to hypoxia. conclusion:taken together, these results indicate that fa antioxidant effects could partly be involved in inhibition of p38 mapk pathway and apoptosis through scavenging ros in hypoxia-stressed pc12 cells.
منابع مشابه
Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways
Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH r...
متن کاملFerulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways
OBJECTIVES Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. MATERIALS AND METHODS We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. RESULTS FA increased cell viability, prevented membrane damage (LDH...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۸، شماره ۵، صفحات ۴۷۸-۴۸۴
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